To monitor relative changes in regional cerebral blood flow during ischemia and reperfusion we used a laser-Doppler flowmeter (ADInstruments) equipped with a pencil probe affixed to the animal’s skull [12 (link)]. Pre-ischemic, intra-ischemic and during first 10 min of recovery rCBF was recorded in both WT and CD38KO animals.
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Monitoring Regional Cerebral Blood Flow
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Other organizations : VA Maryland Health Care System, University of Maryland, Baltimore
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Variable analysis
independent variables
- Genotype (WT vs. CD38KO)
- Relative changes in regional cerebral blood flow (rCBF) during ischemia and reperfusion
- Laser-Doppler flowmeter (ADInstruments) equipped with a pencil probe affixed to the animal's skull
- Pre-ischemic, intra-ischemic and during first 10 min of recovery rCBF
- Positive control: Monitoring rCBF in WT animals
- Negative control: Monitoring rCBF in CD38KO animals
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