Generation of Swap70-/- x 2D2 x Foxp3GFP Mice

Foxp3^GFP mice (38 (link)) were crossed to Swap-70^-/- mice (39 (link)) to generate Swap70^-/- x Foxp3^GFP mice. Foxp3^RFP/GFP mice expressing the Cre recombinase GFP (GFP-Cre) fusion protein as a Foxp3 BAC transgene and the RFP reporter from an internal ribosome entry site (IRES) downstream of the Foxp3 coding region (Foxp3^IRES-RFP) have been previously described (40 (link)). Foxp3^GFP mice were bred to 2D2 mice (Jackson Laboratory), expressing the transgenic, self-reactive Vα3.2 (2D2) T cell receptor (TCR) specific for myelin oligodendrocyte glycoprotein [MOG, (41 (link))] to generate Foxp3^GFP x 2D2-MOG mice. Foxp3^GFP x 2D2-MOG mice were then bred with Swap70^-/- mice to obtain Swap70^-/- x 2D2 x Foxp3^GFP mice. All mice were on C57/BL6 background and were housed and bred either at the animal facility of the CRTD, TU Dresden or the Experimental Center of the Medizinisch-Theoretisches Zentrum, TU Dresden. Animal experiments were performed as approved by the Regierungspräsidium Dresden (AZ 24-9168.24-1/2014-5, 24-9168.24-1/2014-1).