ChIP-seq and ChIP-exo Protocols for Mapping AR and AR-V7 Binding

ChIP assays were performed as previously described^{25 (link)} with the modifications using AR antibody (ActiveMotif, Carlsbad, CA) or AR C-19 (Santa Cruz Biotechnology, Dallas, TX) as described in the Supplementary methods (SMethods). Data generated by Active Motif using the ChIP-exo method and their AR antibody (ActiveMotif, Carlsbad, CA)^{53 (link)} were used to map AR and AR-V7 binding ChIP-seq and ChIP-exo (STable 1) were analysed as described in SMethods, using the bowtie2 (version 2.3, default parameters) alignment software^{54 (link)} and peaks were called using macs2 (version 2.1; − q < 0.05, otherwise mentioned)^{55 (link)}.

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Basil P., Robertson M.J., Bingman WE I.I.I., Dash A.K., Krause W.C., Shafi A.A., Piyarathna B., Coarfa C, & Weigel N.L. (2022). Cistrome and transcriptome analysis identifies unique androgen receptor (AR) and AR-V7 splice variant chromatin binding and transcriptional activities. Scientific Reports, 12, 5351.

Publication 2022

AR antibody AR C-19

Antibody Chip assays Chip exo Chip seq

Corresponding Organization : Baylor College of Medicine

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Variable analysis

independent variables

ChIP assays
AR antibody (ActiveMotif, Carlsbad, CA)
AR C-19 (Santa Cruz Biotechnology, Dallas, TX)

dependent variables

AR and AR-V7 binding ChIP-seq and ChIP-exo

control variables

ChIP assays were performed as previously described^{25 (link)} with the modifications

controls

Positive control: Data generated by Active Motif using the ChIP-exo method and their AR antibody (ActiveMotif, Carlsbad, CA)^{53 (link)}
Negative control: Not explicitly mentioned

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