Antibodies for Cellular Localization

Generation of pericentrin antibodies and Borg5 rabbit and chicken antibodies were described previously (Liu and Zheng, 2009 (link); Vong et al., 2010 (link)). All the other antibodies and molecules used in this study were commercially available, including α-tubulin (T9026, 1:1000; Sigma-Aldrich), actin (A3853, 1:200; Sigma-Aldrich), GM130 (610822, 1:200; BD Biosciences, Franklin Lakes, NJ), isolectin β4 (I21412, 1:200; Invitrogen), Nkx2.5 (sc-8697, 1:50; Santa Cruz Biotechnology, Santa Cruz, CA), PECAM-1 (550274, 1:10; BD Biosciences), phalloidin (A22287 or A12379, 1:200; Invitrogen), phospho-MLC (3674, 1:200; Cell Signaling Technology, Danvers, MA), Sept7 (18991, 1:1000; IBL, Minneapolis, MN), and VE-cadherin (sc-6458, 1:50; Santa Cruz Biotechnology).

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Liu Z., Vong Q.P., Liu C, & Zheng Y. (2014). Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells. Molecular Biology of the Cell, 25(6), 841-851.

Publication 2014

α-tubulin GM130 PECAM-1 phospho-MLC VE-cadherin

Actin Antibodies Chicken Isolectin Pecam 1 Pericentrin Phalloidin Rabbit Ve cadherin α tubulin

Corresponding Organization :

Other organizations : Carnegie Institution for Science, Department of Embryology, National Heart Lung and Blood Institute, National Institutes of Health

Top 5 similar protocols

Variable analysis

independent variables

Generation of pericentrin antibodies
Generation of Borg5 rabbit and chicken antibodies

dependent variables

Outcomes/measurements related to the use of the generated antibodies

control variables

α-tubulin (T9026, 1:1000; Sigma-Aldrich)
Actin (A3853, 1:200; Sigma-Aldrich)
GM130 (610822, 1:200; BD Biosciences, Franklin Lakes, NJ)
Isolectin β4 (I21412, 1:200; Invitrogen)
Nkx2.5 (sc-8697, 1:50; Santa Cruz Biotechnology, Santa Cruz, CA)
PECAM-1 (550274, 1:10; BD Biosciences)
Phalloidin (A22287 or A12379, 1:200; Invitrogen)
Phospho-MLC (3674, 1:200; Cell Signaling Technology, Danvers, MA)
Sept7 (18991, 1:1000; IBL, Minneapolis, MN)
VE-cadherin (sc-6458, 1:50; Santa Cruz Biotechnology)

positive controls

Generation of pericentrin antibodies
Generation of Borg5 rabbit and chicken antibodies

negative controls

Not explicitly mentioned

Annotations

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