Quantifying HIV Reservoir in CD4+ T Cells

The size of the HIV reservoir was estimated by measuring the frequency of CD4+ T cells harboring integrated HIV DNA, as previously described.^{30 (link)} Briefly, CD4+ T cells were isolated from cryopreserved PBMCs by negative selection (StemCell) and subjected to an Alu gnested-PCR to quantify the number of integrated HIV genomes.

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Tawakol A., Ishai A., Li D., Takx R.A., Hur S., Kaiser Y., Pampaloni M., Rupert A., Hsu D., Sereti I., Fromentin R., Chomont N., Ganz P., Deeks S.G, & Hsue P.Y. (2017). Arterial and Lymph Node Inflammation Associate with Distinct Inflammatory Pathways in HIV. JAMA cardiology, 2(2), 163-171.

Publication 2017

negative selection

Cd4 t cells Genomes Stemcell

Corresponding Organization : University of California, San Francisco

Other organizations : Massachusetts General Hospital, Harvard University, Academic Medical Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Centre Hospitalier de l’Université de Montréal

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Variable analysis

independent variables

Isolation of CD4+ T cells from cryopreserved PBMCs by negative selection

dependent variables

Frequency of CD4+ T cells harboring integrated HIV DNA

control variables

Alu gnested-PCR to quantify the number of integrated HIV genomes

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