SDS-PAGE and Western blotting analysis were performed as described (1 (link)). The following antibody concentrations were used: mouse monoclonal anti-FLAG (Sigma, M2), 1:3,000; mouse monoclonal anti-GFP (Roche Applied Science), 0.1 μg/ml; and Alexa Fluor680-conjugated anti-mouse secondary antibody (Molecular Probes), 1:5,000. Blots were scanned and quantified using the Odyssey imaging system (LI-COR Biosciences).
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