Generating T. gondii cDNA Library

Toxoplasma gondii (Type II) PRU strain was selected for generating a cDNA library because it encodes the TgGRA15, which allows type II strains of T. gondii to be a highly inducer of NF-kB activation, compared to type I strains [28 (link)] and type III strains [11 (link)]. The Type II PRU strain was maintained in our laboratory by the passage of cysts in female BALB/c mice (4 to 5 weeks of age). Mice were purchased from the Center of Experimental Animals, Lanzhou University, Lanzhou, China. Parasite maintenance in the mice experiment was performed in strict compliance with state and institutional animal care guidelines. The tissue cysts of T. gondii PRU strain were harvested from infected mice 30 days post-infection and were used for total RNA extraction.