COVID-19 Lymphocyte Subset Analysis

Lymphocyte subset percentages were analyzed before initial treatment. EDTA anticoagulated peripheral blood (2 ml) were collected from patients with COVID-19 on admission (18 (link)). All samples were tested within 6 h of being obtained. Briefly, CD3+ total T-lymphocytes, CD19+ B-cell, CD3+/CD4+/CD8+ T-cell, and CD16+CD56+ NK-cell counts (cells/μl) were measured by multiple-color flow cytometry with human monoclonal anti-CD19-PE, anti-CD3-fluorescein isothiocyanate (FITC), anti-CD4-phycoerythrin (PE), anti-CD8-allophycocyanin (APC), anti-CD16-APC, and anti-CD56-PE antibodies (BD Multitest) according to the manufacturer's instructions. The cells were analyzed on a Partec Cube 6 Flow cytometry system (Sysmex Europe GmbH, Germany).

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Shokati Eshkiki Z., Shahriari A., Seyedtabib M., Torabizadeh M., Assarehzadegan M.A., Nashibi R., Khosravi M., Neisi N., Mard S.A, & Shayesteh A.A. (2021). Innate and Adaptive Immunity Imbalance With Severe COVID-19 Pneumonia in Children and Adults. Frontiers in Pediatrics, 9, 736013.

Publication 2021

anti-CD19-PE anti-CD4-phycoerythrin anti-CD8-allophycocyanin anti-CD16-APC anti-CD56-PE antibodies

Allophycocyanin Anti antibodies Anti cd3 B cell Blood Cd4 t cell Cells Covid 19 Edta Flow cytometry Fluorescein Human Isothiocyanate Patients Phycoerythrin T lymphocytes

Corresponding Organization : Ahvaz Jundishapur University of Medical Sciences

Other organizations : Iran University of Medical Sciences

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

CD3+ total T-lymphocytes (cells/μl)
CD19+ B-cell (cells/μl)
CD3+/CD4+ T-cell (cells/μl)
CD3+/CD8+ T-cell (cells/μl)
CD16+CD56+ NK-cell (cells/μl)

control variables

EDTA anticoagulated peripheral blood (2 ml) from patients with COVID-19 on admission
All samples were tested within 6 h of being obtained

controls

No positive or negative controls were explicitly mentioned in the provided information.

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