Orthotopic DIPG Tumor Xenograft Model
Corresponding Organization : Princess Máxima Center
Other organizations : Heidelberg University, University Hospital Heidelberg, University Medical Center Utrecht
Variable analysis
- Injection of eGFP-lucF-gene-positive HSJD-DIPG-007 cells
- Possible signs of stress and postoperative complications (lack of food/water intake, antisocial behaviour, and motor deficits)
- Carprofen administration in drinking water for 24 hours before and after injection
- Subcutaneous carprofen administration 30 minutes before surgery for local pain management
- Anesthesia with isoflurane
- Fixing mice on a stereotactic frame with bite and ear bars
- Application of eye cream to prevent eye damage
- Keeping mice warm during the procedure
- Lidocaine application before removal of the fascia on the skull
- Injection of HSJD-DIPG-007 cells suspended in PBS or Matrigel at a rate of 2 μL/min
- Leaving the needle in place for 7 minutes before slow retraction
- Wound closure with topical skin adhesive Histoacryl
- Placing mice under a heating lamp until awake
- Positive control: Not specified
- Negative control: Not specified
Annotations
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