Serum and peritoneal lavage from animals were used for cytokine dosing by flow cytometry. The cytometric bead array (CBA) technique was used for the quantification of TNF-α, MCP-1, IL-6, IL-10, IL-12, and IFN-γ [40 (link)], using a mouse inflammation cytokine kit (Becton Dickinson Biosciences, San Jose, CA, EUA).
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