Identifying and Analyzing Brain-Associated Macrophages

BAM were identified by well-established criteria, including expression of CD206, ability to phagocytize dextran and perivascular location [24 (link), 25 (link), 40 (link), 41 (link)]. The association with cortical blood vessels was confirmed by co-labeling with the endothelial marker Glut-1 (rabbit polyclonal, 1:200, Calbiochem), α-Actin (rabbit polyclonal, 1:300, abcam), or DiO [25 (link), 39 (link)]. For CD206⁺ BAM, randomly selected fields (20 × objective; 4 confocal images/mouse; n = 5 mice/group) within the somatosensory cortex were analyzed. For dextran⁺ BAM, a representative coronal section from each mouse was reconstructed from tiled images taken with the confocal microscope, and the whole somatosensory cortex (n = 5/group) was analyzed. ImageJ (NIH) was used for all image analyses.

Free full text: Click here

Uekawa K., Hattori Y., Ahn S.J., Seo J., Casey N., Anfray A., Zhou P., Luo W., Anrather J., Park L, & Iadecola C. (2023). Border-associated macrophages promote cerebral amyloid angiopathy and cognitive impairment through vascular oxidative stress. Molecular Neurodegeneration, 18, 73.

Publication 2023

α-Actin

Actin Blood vessels Confocal microscope Cortical Dextran Endothelial Glut 1 Mouse Rabbit Somatosensory cortex

Corresponding Organization :

Other organizations : Cornell University, MIND Research Institute

Top 5 similar protocols

Variable analysis

independent variables

Expression of CD206
Ability to phagocytize dextran
Perivascular location

dependent variables

Percentage of CD206+ BAM in randomly selected fields within the somatosensory cortex
Percentage of dextran+ BAM in the whole somatosensory cortex

control variables

Endothelial marker Glut-1
α-Actin

controls

Positive control: Co-labeling with endothelial marker Glut-1, α-Actin, or DiO to confirm the association of BAM with cortical blood vessels
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!