Tyrosine Kinase Activity Assay of rSmFGFRA

A Universal Tyrosine Kinase Assay Kit (Takara, Melbourne, Australia) was used to determine the enzymatic activity of rSmFGFRA at different concentrations (100 ng/μl - 0.78 ng/μl) in the presence or absence of 5 μM and 10 μM BIBF 1120 as described (14 (link), 36 (link)), following the manufacturer’s instructions. DMSO (0.1%) was served as negative control. The activity of rSmFGFRA was determined by comparing its absorbance with that of control protein tyrosine kinase (PTK), supplied with the kit, according to the manufacturer’s instructions. Technical duplicates were performed and the experiment was repeated twice.

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Du X., McManus D.P., Fogarty C.E., Jones M.K, & You H. (2022). Schistosoma mansoni Fibroblast Growth Factor Receptor A Orchestrates Multiple Functions in Schistosome Biology and in the Host-Parasite Interplay. Frontiers in Immunology, 13, 868077.

Publication 2022

Universal Tyrosine Kinase Assay Kit

Assay Bibf 1120 Dmso Enzymatic activity Protein tyrosine kinase

Corresponding Organization : QIMR Berghofer Medical Research Institute

Other organizations : University of Queensland, University of the Sunshine Coast

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Variable analysis

independent variables

Concentration of rSmFGFRA (100 ng/μl - 0.78 ng/μl)
Presence or absence of BIBF 1120 (5 μM and 10 μM)

dependent variables

Enzymatic activity of rSmFGFRA

control variables

DMSO (0.1%) as negative control

controls

Positive control: Control protein tyrosine kinase (PTK) supplied with the kit
Negative control: DMSO (0.1%)

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