Non-invasive Evaluation of Inflammation Markers

For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [22 (link)], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [23 (link)]. Zonulin was measured using a human zonulin ELISA kit (Elabscience, Wuhan, China) [24 (link)]. All assays were conducted according to the manufacturer’s instructions. The serum samples were collected at baseline and stored at −80 °C until analyses.

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Fujinaga Y., Namisaki T., Tsuji Y., Suzuki J., Murata K., Takeda S., Takaya H., Inoue T., Noguchi R., Fujimoto Y., Enomoto M., Nishimura N., Kitagawa K., Kaji K., Kawaratani H., Akahane T., Mitoro A, & Yoshiji H. (2022). Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis. International Journal of Molecular Sciences, 23(17), 9814.

Publication 2022

human zonulin ELISA kit

Assays Enzyme linked immunosorbent assay Human Serum Zonulin

Corresponding Organization : Nara Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum levels of sCD163
Serum levels of sMR
Serum levels of zonulin

control variables

Serum samples were collected at baseline and stored at -80°C until analyses

controls

No positive or negative controls were explicitly mentioned

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