Protocol detail

Drosophila Genetic Stock Maintenance

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Drosophila stocks were maintained on standard cornmeal-molasses food at 25°C. The PINK1-myc transgenic line, UAS-PINK1 transgenic line, UAS-Miro-myc transgenic line, Mhc-GAL4 driver line and Dmef2-GAL4 driver line have been previously described [20] (link), [34] (link), [44] (link), [45] (link), [46] (link). The following lines were obtained from the Bloomington Drosophila Stock Center: elav-GAL4, ey-GAL4, sqh-mito-EYFP, Lon^G3998, Df(3L)Exel9011, P{VALIUM20-mCherry}attP2 (Control-RNAi), and P{TRiP.HMS01060}attP2 (Lon-RNAi2). The P{GD11336}-v21860 (PINK1-RNAi), P{KK101663}-v109629 (AFG3L2-RNAi1), and P{GD14030}-v36036 (Lon-RNAi1) RNAi lines were obtained from the Vienna Drosophila Resource Center. All other RNAi lines tested were obtained from the stock centers indicated in Table S1.

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Thomas R.E., Andrews L.A., Burman J.L., Lin W.Y, & Pallanck L.J. (2014). PINK1-Parkin Pathway Activity Is Regulated by Degradation of PINK1 in the Mitochondrial Matrix. PLoS Genetics, 10(5), e1004279.

Publication 2014

elav-GAL4 ey-GAL4

Drosophila Food Mito Molasses Rnai Transgenic

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Variable analysis

independent variables

PINK1-myc transgenic line
UAS-PINK1 transgenic line
UAS-Miro-myc transgenic line
Lon^G3998
Df(3L)Exel9011
P{VALIUM20-mCherry}attP2 (Control-RNAi)
P{TRiP.HMS01060}attP2 (Lon-RNAi2)
P{GD11336}-v21860 (PINK1-RNAi)
P{KK101663}-v109629 (AFG3L2-RNAi1)
P{GD14030}-v36036 (Lon-RNAi1)

dependent variables

Not explicitly mentioned

control variables

Maintained on standard cornmeal-molasses food at 25°C
Mhc-GAL4 driver line
Dmef2-GAL4 driver line
elav-GAL4
ey-GAL4
sqh-mito-EYFP

positive controls

Not explicitly mentioned

negative controls

P{VALIUM20-mCherry}attP2 (Control-RNAi)

Annotations

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