TZM-bl assay was performed as described [30 (link), 36 (link)]. Briefly, 10 μl of virus supernatant was inoculated into TZM-bl cells in 96-well plate (Nunc), and the β-galactosidase activity was measured by using the Galacto-Star mammalian reporter gene assay system (Thermo Fisher Scientific) and a 2030 ARVO X multi-label counter instrument (PerkinElmer) according to the manufacturers’ procedure. The relative infectivity was determined by relative light unit of this assay.
Nakano Y., Yamamoto K., Ueda M.T., Soper A., Konno Y., Kimura I., Uriu K., Kumata R., Aso H., Misawa N., Nagaoka S., Shimizu S., Mitsumune K., Kosugi Y., Juarez-Fernandez G., Ito J., Nakagawa S., Ikeda T., Koyanagi Y., Harris R.S, & Sato K. (2020). A role for gorilla APOBEC3G in shaping lentivirus evolution including transmission to humans. PLoS Pathogens, 16(9), e1008812.
Assay used (Galacto-Star mammalian reporter gene assay system)
Instrument used (2030 ARVO X multi-label counter)
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
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