Western Blot Analysis of Metabolic Proteins

Western blot was performed as previously described [16 (link)]. After incubation with the secondary antibody, the proteins were detected by chemiluminescence. Primary antibodies used in this study were anti-HMGCS2 (for WB) (1:2000; Abcam ab137043), anti-α-SMA (1:2000; Abcam, ab7817), anti-Collagen1 (1:1000; Cell Signaling Technology, #72026), anti-Fibronectin (1:2000; Cell Signaling Technology, #26836), anti-PPARα (1:1000; Affinity, AF5301), anti-CPT1A (1:1000; Affinity, AF5301), anti-CPT2 (1:1000; Affinity, AF5301), and anti-GAPDH (1:4000; Beyotime, China).

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Yang J., Pan X., Xu M., Li Y., Liang C., Liu L., Li Z., Wang L, & Yu G. (2024). Downregulation of HMGCS2 mediated AECIIs lipid metabolic alteration promotes pulmonary fibrosis by activating fibroblasts. Respiratory Research, 25, 176.

Publication 2024

anti-α-SMA anti-Fibronectin AF5301 anti-GAPDH

Corresponding Organization :

Other organizations : Henan Normal University

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Variable analysis

independent variables

Primary antibodies used in this study were anti-HMGCS2 (for WB) (1:2000; Abcam ab137043), anti-α-SMA (1:2000; Abcam, ab7817), anti-Collagen1 (1:1000; Cell Signaling Technology, #72026), anti-Fibronectin (1:2000; Cell Signaling Technology, #26836), anti-PPARα (1:1000; Affinity, AF5301), anti-CPT1A (1:1000; Affinity, AF5301), and anti-CPT2 (1:1000; Affinity, AF5301)

dependent variables

Protein detection by chemiluminescence

control variables

Western blot was performed as previously described [16 (link)]
Anti-GAPDH (1:4000; Beyotime, China)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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