Synthesis and Characterization of PBAEs

PBAEs were synthesized as described previously.19 (link) Briefly, backbone monomers, 1,4-Butanediol diacrylate (B4, Alfa Aesar, Haverhill, MA) or 1,5-Pentanediol diacrylate (B5, Santa Cruz Biotechnology, Dallas, TX), were mixed at a 1.1:1 ratio with sidechain monomers 3-amino-1-propanol (S3, Alfa Aesar, Haverhill, MA), 4-amino-1-butanol (S4, Fisher Scientific, Hampton, NH), or 5-amino-1-pentanol (S5, Alfa Aesar, Haverhill, MA) and allowed to react overnight at 85°C. B-S polymers were then resuspended in tetrahydrofuran (THF) and endcapped with 2-(3-Aminopropylamino)ethanol (E6, Sigma, St. Louis, MO) or 1-(3-Aminopropyl)-4-methylpiperazine (E7, Alfa Aesar, Haverhill, MA) for 2 h at room temperature. Resulting polymers B4-S4-E6 (446), B4-S5-E7 (457), and B5-S3-E6 (536) were ether precipitated, dried, and stored at −20°C on desiccant. To determine the molecular weight and polydispersity index of PBAE 536, ether purified polymers were resuspended in tetrahydrofuran and gel permeation chromatography was performed using polystyrene reference standards and a refractive index detector (Agilent, Santa Clara, CA).

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Vaughan H.J., Zamboni C.G., Luly K.M., Li L., Gabrielson K.L., Hassan L.F., Radant N.P., Bhardwaj P., Selaru F.M., Pomper M.G, & Green J.J. (2023). Non-Viral Gene Delivery to Hepatocellular Carcinoma via Intra-Arterial Injection. International Journal of Nanomedicine, 18, 2525-2537.

Publication 2023

1,4-Butanediol diacrylate (B4 3-amino-1-propanol 5-amino-1-pentanol 1-(3-Aminopropyl)-4-methylpiperazine refractive index detector

1 propanol Backbone Butanediol diacrylate Butanol Desiccant Ethanol Ether Gel permeation chromatography Polymers Polystyrene Tetrahydrofuran

Corresponding Organization :

Other organizations : Johns Hopkins Medicine, Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center

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Variable analysis

independent variables

Backbone monomers (1,4-Butanediol diacrylate (B4) or 1,5-Pentanediol diacrylate (B5))
Sidechain monomers (3-amino-1-propanol (S3), 4-amino-1-butanol (S4), or 5-amino-1-pentanol (S5))
Endcapping agents (2-(3-Aminopropylamino)ethanol (E6) or 1-(3-Aminopropyl)-4-methylpiperazine (E7))

dependent variables

Molecular weight of PBAE 536
Polydispersity index of PBAE 536

control variables

Reaction time (overnight at 85°C)
Resuspension in tetrahydrofuran (THF)
Endcapping time (2 h at room temperature)
Ether precipitation, drying, and storage conditions (-20°C on desiccant)

controls

Polystyrene reference standards used for gel permeation chromatography

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