RNA was isolated and prepared as described in detail in19 (link). In brief, pieces of 50 mg of liver tissue were homogenized in RNAeasy lysis buffer (Qiagen, Hilden, Germany) with 1% 2-mercaptoethanol. Total RNA was then isolated according to the manufacturer’s protocol (RNeasy, Qiagen, Hilden, Germany).
RNA concentrations and purity have been determined using a NanoDrop ND-1000 UV–Vis Spectrophotometer (Thermo Scientific, Karlsruhe, Germany) at 260 nm and 260/280 nm ratio, respectively. All samples were stored at −80 °C before further analysis by RNA-Seq.
RNA concentrations and purity have been determined using a NanoDrop ND-1000 UV–Vis Spectrophotometer (Thermo Scientific, Karlsruhe, Germany) at 260 nm and 260/280 nm ratio, respectively. All samples were stored at −80 °C before further analysis by RNA-Seq.
Free full text: Click here
