Freshly excised liver tissues were fixed in 10% formalin (Sigma, Saint Louis, MO) overnight. The tissue was then embedded in paraffin, cut and mounted on slides by the University of Colorado Anschutz Medical Campus Histology Core. Standard hematoxylin and eosin (H&E) staining was performed. Immunohistochemistry was performed using the ABC citrate antigen retrieval system (Vector labs, Burlingame, CA) and by incubating liver tissue sections with primary antibodies raised against CYP2E1 (AB1252; Millipore, Darmstadt, Germany), SOD2 (ab13534; Abcam, Cambridge, MA, USA), and SOD2 acetyl-K68 (ab137037; Abcam, Cambridge, MA, USA) overnight at 4 °C via a protocol described by Harris et. al. (Harris et al., 2015 (link)). Histologic images were captured on an Olympus BX51 microscope equipped with a 4 megapixel Macrofire digital camera using the PictureFrame Application 2.3 (Optronics). All images were cropped and assembled using Photoshop CS2 (Adobe Systems, Inc.). Images were then imported into Slidebook (3I, Denver CO) for quantification, which was performed on 5 images per animal, 3–4 animals per group.