Jumonji Inhibitor Compound Preparation

Chloroquine (Sigma-Aldrich) was
dissolved in H₂O, whereas all other drugs were dissolved
in DMSO (Sigma-Aldrich). The Jumonji inhibitors used in this study
were obtained from Cayman Chemical, Ann Arbor, MI (GSK-J4, GSK-J5,
and CPI-455), Selleck Chemicals, Houston, TX (ML324), and Xcess Biosciences,
San Deigo, CA (KDM5-C70 and SD-70). JIB-04 E and Z isomers were synthesized
as previously described.^{23 (link)} Compounds freshly
dissolved in DMSO were aliquoted to minimize freeze/thaw cycles and
stored at −20 °C.

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Matthews K.A., Senagbe K.M., Nötzel C., Gonzales C.A., Tong X., Rijo-Ferreira F., Bhanu N.V., Miguel-Blanco C., Lafuente-Monasterio M.J., Garcia B.A., Kafsack B.F, & Martinez E.D. (2020). Disruption of the Plasmodium falciparum Life Cycle through Transcriptional Reprogramming by Inhibitors of Jumonji Demethylases. ACS Infectious Diseases, 6(5), 1058-1075.

Publication 2020

Chloroquine DMSO GSK-J4 CPI-455

Cayman Chloroquine Dmso Drugs Freeze Gsk j4 Inhibitors Isomers Jib 04 Ml324

Corresponding Organization : The University of Texas Southwestern Medical Center

Other organizations : Cornell University, University of Pennsylvania, GlaxoSmithKline (Spain)

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Variable analysis

independent variables

Chloroquine (Sigma-Aldrich)
Jumonji inhibitors: GSK-J4, GSK-J5, CPI-455 (Cayman Chemical), ML324 (Selleck Chemicals), KDM5-C70 and SD-70 (Xcess Biosciences)
JIB-04 E and Z isomers (synthesized as previously described)

dependent variables

Unspecified

control variables

DMSO (Sigma-Aldrich) as a solvent for all drugs except Chloroquine

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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