TEWL was measured using the Tewameter TM300 (probe 06030433, Courage & Khazaka, Cologne, Germany). Tape-stripping consisted of 3 repetitions of adhesive tape application (Scotch Magic tape, 3M, St. Paul, MN) performed one week following hair removal. Epidermal abrasion was induced by twice repeated shaving of ventral skin (Personna American Safety Razor Co., Verona, VA). Ear thickness following a single application of TPA (Sigma, 0.5 nmoles) was measured using an engineer’s micrometer (Mitutoyo 7301). Epidermal hypertrophy was induced by TPA (20 nmoles) application 2x/wk for 4 wks. Histologic examination and measurement of minimal epidermal thickness was performed as previously described (Lewis et al., 2014 (link)),
Protocol detail
Skin Barrier Assessment and Epidermal Irritation
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Corresponding Organization : Yale University
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Variable analysis
independent variables
- Tape-stripping (3 repetitions of adhesive tape application)
- Epidermal abrasion (twice repeated shaving of ventral skin)
- TPA application (0.5 nmoles for ear thickness measurement, 20 nmoles for epidermal hypertrophy induction)
- TEWL (measured using Tewameter TM300)
- Ear thickness (measured using an engineer's micrometer)
- Epidermal thickness (measured histologically)
- Tape type (Scotch Magic tape, 3M)
- Shaver (Personna American Safety Razor Co.)
- TPA source (Sigma)
- Epidermal hypertrophy induced by 20 nmoles TPA application 2x/wk for 4 weeks
- None explicitly mentioned
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