Mouse experiments were carried out after approval of the institutional review board (Ethical Committee of the UAM, CEI-101-1891-A325; CM PROEX 233/19) in compliance with animal policies and ethical guidelines of the European Community. Mice were housed in the Animal Facility of the CBMSO with a 12-h light/12-h dark cycle and temperatures of 18–23°C with 40–60% humidity.
Conditional IF1 knockout mice (Atp5if1-KO) in T lymphocytes (CD4+ IF1-KO) were obtained by breeding the IF1-floxed mice25 (link) with the B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (The Jackson Laboratory) mouse line. The latter expresses the Cre recombinase in CD4+ and CD8+ T lymphocytes. Mice were maintained on C57BL/6J background. Two-Three month-old male and female mice were used except in ageing experiments and otherwise indicated. Wild-type and IF1-floxed alleles were distinguished with 5′-TGCCTGACATTGGTATTGGG-3′ and 5′-GTGCAGCTTGTGGGAGTCAG-3′ primers.25 (link) The transgene encoding the Cre recombinase was detected with 5′-CAATTTACTGACCGTACAC-3′ and 5′-TAATCGCCATCTTCC AGCAG-3′ primers. No influence of gender is reported.
Conditional IF1 knockout mice (Atp5if1-KO) in T lymphocytes (CD4+ IF1-KO) were obtained by breeding the IF1-floxed mice25 (link) with the B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (The Jackson Laboratory) mouse line. The latter expresses the Cre recombinase in CD4+ and CD8+ T lymphocytes. Mice were maintained on C57BL/6J background. Two-Three month-old male and female mice were used except in ageing experiments and otherwise indicated. Wild-type and IF1-floxed alleles were distinguished with 5′-TGCCTGACATTGGTATTGGG-3′ and 5′-GTGCAGCTTGTGGGAGTCAG-3′ primers.25 (link) The transgene encoding the Cre recombinase was detected with 5′-CAATTTACTGACCGTACAC-3′ and 5′-TAATCGCCATCTTCC AGCAG-3′ primers. No influence of gender is reported.
Free full text: Click here
