Aniline Blue Staining of Maize Leaves

For aniline blue staining, the maize leaves were bleached using ethanol until the samples were transparent. Next, 150 mM K₂HPO₄ was vacuum infiltrated in the leaves to replace the extra ethanol three times every 10 min. After removing the buffer, samples were covered with 0.01% aniline blue solution (w/v in 150 mM K₂HPO₄) at room temperature and maintained in the dark for 2 h. Confocal microscopy (Leica, Germany) was carried out with DAPI channel (Emission 490–520 nm).

Free full text: Click here

Gu X., Cao Z., Li Z., Yu H, & Liu W. (2024). Plant immunity suppression by an β-1,3-glucanase of the maize anthracnose pathogen Colletotrichum graminicola. BMC Plant Biology, 24, 339.

Publication 2024

Confocal microscopy

Corresponding Organization :

Other organizations : Hebei Agricultural University, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Top 5 similar protocols

Variable analysis

independent variables

Concentration of aniline blue solution (0.01% w/v in 150 mM K2HPO4)

dependent variables

Fluorescence signal detected using DAPI channel (Emission 490–520 nm) in confocal microscopy

control variables

Temperature (room temperature)
Duration of aniline blue staining (2 hours)
Vacuum infiltration of 150 mM K2HPO4 (3 times every 10 minutes)

controls

No positive control was explicitly mentioned.
No negative control was explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!