Biomarker Analysis in Sepsis Diagnosis

Complete blood counts (CBCs) were performed on whole blood by Sysmex XE-9000 (Dasit, Italy) following the manufacturer’s instruction. NLR, PLR, and MLR were calculated by the ratio between absolute values of neutrophils, lymphocytes, monocytes, respectively, and that of platelets.
CRP protein was measured by Alinity c (Abbott, diagnostics) following the manufacturer’s instruction.
PCT and MR-proADM plasma concentrations were measured by an automated Kryptor analyzer, using a time-resolved amplified cryptate emission (TRACE) technology assay (Kryptor PCT; Brahms AG; Hennigsdorf, Germany) with commercially available immunoluminometric assays (Brahms) [5 (link),21 (link),25 (link),26 (link)].
Blood specimens from patients were collected in BACTEC bottles containing anaerobic or aerobic broth and resins. Blood culture bottles were incubated in BACTEC FX instrument (Becton Dickinson, Meylan, France) until they were positive for bacterial growth or for a maximum of 5 days. Positive samples were cultivated in selective agar media. Growing colonies were identified by MALDI-TOF (Brahms) [5 (link),21 (link),25 (link),26 (link)]. Selective and non-selective media were used for microbiological cultures.

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Spoto S., Lupoi D.M., Valeriani E., Fogolari M., Locorriere L., Beretta Anguissola G., Battifoglia G., Caputo D., Coppola A., Costantino S., Ciccozzi M, & Angeletti S. (2021). Diagnostic Accuracy and Prognostic Value of Neutrophil-to-Lymphocyte and Platelet-to-Lymphocyte Ratios in Septic Patients outside the Intensive Care Unit. Medicina, 57(8), 811.

Publication 2021

Alinity c BACTEC FX instrument

Aerobic Agar Assays Bacterial Blood Blood culture Cbcs Cryptate Diagnostics Lymphocytes Maldi Monocytes Neutrophils Patients Plasma Platelets Protein Resins

Corresponding Organization : Università Campus Bio-Medico

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Complete blood counts (CBCs)
NLR (neutrophil-to-lymphocyte ratio)
PLR (platelet-to-lymphocyte ratio)
MLR (monocyte-to-lymphocyte ratio)
CRP (C-reactive protein)
PCT (procalcitonin)
MR-proADM (mid-regional pro-adrenomedullin)

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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