Immunostaining of Hypopharyngeal Cancer

Tumor specimens were obtained from 38 consecutive cases from 2013 to 2017 of hypopharyngeal cancer at Miyagi Cancer Center (Natori, Japan), in which patients underwent biopsy before treatment. All of the cases were pathologically diagnosed as squamous cell carcinoma. Immunostaining of formalin-fixed paraffin-embedded tissue was performed as previously described^{8 (link)} using the following antibodies: anti-CD271 (1:2500; C40-1457; BD Biosciences), anti-phosphorylated (p)-RELA (1:1500; ab86299; Abcam, Cambridge, UK), and EnVision + Dual Link System-HRP (anti-mouse and anti-rabbit; Dako, Glostrup, Denmark). Heat-induced epitope retrieval was performed by microwaving the sections in target retrieval solution for CD271 staining (pH 9.0; Dako) or Immunosaver (Fujifilm Wako Pure Chemical Corp., Osaka, Japan) for p-RELA staining.

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Nakazato A., Mochizuki M., Shibuya-Takahashi R., Fujimori H., Fujii K., Saijoh S., Morita S., Yamazaki T., Imai T., Sato I., Satoh K., Yamaguchi K., Sugamura K., Yasuda J., Matsuura K., Shojaku H., Asada Y, & Tamai K. (2022). RELA is required for CD271 expression and stem-like characteristics in hypopharyngeal cancer. Scientific Reports, 12, 17751.

Publication 2022

C40-1457 ab86299 EnVision + Dual Link System-HRP Immunosaver

Antibodies Biopsy Cancer Cd271 Embedded paraffin Epitope Formalin Hypopharyngeal cancer Mouse Patients Rabbit Rela Squamous cell carcinoma Tumor

Corresponding Organization :

Other organizations : Miyagi Prefectural Hospital Organization, Tohoku Medical and Pharmaceutical University Hospital, National Cancer Center Hospital East, University of Toyama

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression of CD271
Expression of phosphorylated (p)-RELA

control variables

Tumor specimens from 38 consecutive cases of hypopharyngeal cancer
All cases were pathologically diagnosed as squamous cell carcinoma
Formalin-fixed paraffin-embedded tissue samples were used
Immunostaining was performed as previously described in the referenced study

controls

Positive control: None specified
Negative control: None specified

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