Inhibition
of MERS-CoV pseudovirus infection was assessed using a previously
described method.42 (link) Briefly, the serially
diluted tested peptides were added to a 96-well plate and incubated
with MERS-CoV pseudovirus for 30 min at 37 °C. Then, the pseudovirus/peptide
mixture was added to the Huh-7 cells. Cultures were refed with fresh
medium at 12 h postinfection and incubated for an additional 48 h
at 37 °C. Fluorescence was determined using a luciferase kit
(Promega) and an Ultra 384 luminometer (Tecan).
of MERS-CoV pseudovirus infection was assessed using a previously
described method.42 (link) Briefly, the serially
diluted tested peptides were added to a 96-well plate and incubated
with MERS-CoV pseudovirus for 30 min at 37 °C. Then, the pseudovirus/peptide
mixture was added to the Huh-7 cells. Cultures were refed with fresh
medium at 12 h postinfection and incubated for an additional 48 h
at 37 °C. Fluorescence was determined using a luciferase kit
(Promega) and an Ultra 384 luminometer (Tecan).
