Plasma levels of PfHRP2 were measured to quantify total parasite biomass (38 (link)), using a commercially available ELISA kit (Malaria Ag CELISA kit, catalog KM2, Cellabs). Analyses were performed according to the manufacturer’s instructions, in duplicate for each patient, and absorbance was read at 450 nm using a Multiskan Spectrum ELISA plate reader (Thermo Fisher Scientific).
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