Genotyping of COL22A1 Variants

The buccal cells were collected using Copan FLOQSwabs (Copan Diagnostics, Inc., Murrieta, CA, USA) during post-surgery control. DNA was extracted using a GenElute Mammalian Genomic DNA Miniprep Kit (Sigma, Taufkirchen, Germany) in agreement with the manufacturer’s instructions. On a StepOne Real-Time Polymerase Chain Reaction (RT-PCR) instrument (Applied Biosystems, Bedford, MA, USA), all samples were genotyped in duplicate using the TaqMan^® pre-designed SNP genotyping assays (C_176045196_10 for the COL22A1 rs11784270 and C_29400116_10 for the COL22A1 rs6577958, Applied Biosystems, Bedford, MA, USA) according to the protocol [12 (link)].

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Sun Z., Cięszczyk P., Lulińska E., Dzitkowska-Zabielska M., Johne M., Humińska-Lisowska K., Michałowska-Sawczyn M., Ficek K., Leońska-Duniec A., Mastalerz A., Janczyk A, & Marek S. (2022). Are COL22A1 Gene Polymorphisms rs11784270 and rs6577958 Associated with Susceptibility to a Non-Contact Anterior Cruciate Ligament Injury in Polish Athletes?. International Journal of Environmental Research and Public Health, 20(1), 515.

Publication 2022

Copan FLOQSwabs GenElute Mammalian Genomic DNA Miniprep Kit StepOne Real-Time Polymerase Chain Reaction (RT-PCR) instrument TaqMan® pre-designed SNP genotyping assays

Assays Cells Diagnostics Genomic Mammalian Real time polymerase chain reaction Rt pcr Surgery

Corresponding Organization : Józef Piłsudski University of Physical Education in Warsaw

Other organizations : Gdansk University of Physical Education and Sport, Akademii Wychowania Fizycznego im. Jerzego Kukuczki w Katowicach, University of Szczecin

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Genotype of COL22A1 rs11784270 and COL22A1 rs6577958

control variables

Buccal cells collected using Copan FLOQSwabs
DNA extracted using GenElute Mammalian Genomic DNA Miniprep Kit
Genotyping performed on StepOne Real-Time PCR instrument
Genotyping done in duplicate using TaqMan pre-designed SNP genotyping assays

controls

No positive or negative controls were explicitly mentioned in the provided information.

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