A normal human lung epithelial cell line (BEAS-2B) and NSCLC cell lines (H1650, H460 and A549; all from the China Infrastructure of Cell Line Resource) were cultured in RPMI-1640 basic medium containing 10% fetal bovine serum, 100 mg/ml streptomycin and 100 U/ml penicillin (all from Gibco; Thermo Fisher Scientific, Inc.) in an incubator with 5% CO2 at 37°C.
Short hairpin RNA targeting CYTOR (sh-CYTOR), miR-206 mimics, miR-206 inhibitor, mimics-negative control (NC), inhibitor-NC, overexpression vector for PTMA (pcDNA3.1-PTMA) and NC (empty pcDNA3.1 vector; Shanghai GenePharma Co., Ltd.) were transfected with Lipofectamine® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's protocol to generate stably transfected cells (30 (link)). The plasmids and sequence information are provided inTable SII .
Short hairpin RNA targeting CYTOR (sh-CYTOR), miR-206 mimics, miR-206 inhibitor, mimics-negative control (NC), inhibitor-NC, overexpression vector for PTMA (pcDNA3.1-PTMA) and NC (empty pcDNA3.1 vector; Shanghai GenePharma Co., Ltd.) were transfected with Lipofectamine® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's protocol to generate stably transfected cells (30 (link)). The plasmids and sequence information are provided in
