A dosage of 10 mg/kg bodyweight of NQDI 1 (Tocris Bioscience, Bristol, UK) or an equal volume of vehicle was intraperitoneally (i.p.) administered into mice after CS instillation (10 mice per group). Detailed methods have been outlined in the previous article (17 (link)). Lung tissues were harvested and used for immunohistochemical and western blot analyses after 7- and 56-days exposure to CS.
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