Urinary Biomarkers in Murine Nephropathy

Urine samples were collected from mice individually housed in metabolic cages. Urinary albumin/creatinine ratio (mg/mmol) (Albuwell and Creatinine Companion, Exocell Inc., Philadelphia, PA, USA) was determined biweekly post-ADR injection by ELISA and normalized by urinary creatinine levels as described [10 (link), 34 (link)]. Urinary albumin level was also estimated by Coomassie Blue gel staining of electrophoresed urine samples as reported.[10 (link), 34 (link)] Urinary Ang II /creatinine ratio (pmol/μmol) (Immuno-Biological Laboratories, IBL America, Minneapolis, MN, USA) was assayed by ELISA and normalized by urinary creatinine levels as described.[34 (link), 35 (link)]

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Liao M.C., Miyata K.N., Chang S.Y., Zhao X.P., Lo C.S., El-Mortada M.A., Peng J., Chenier I., Yamashita M., Ingelfinger J.R., Chan J.S, & Zhang S.L. (2022). Angiotensin II Type-2-Receptor Stimulation Ameliorates Focal and Segmental Glomerulosclerosis in Mice. Clinical science (London, England : 1979), 136(10), 715-731.

Publication 2022

Albuwell and Creatinine Companion

Albumin Biological Companion Coomassie blue Creatinine Elisa Mice Urinary

Corresponding Organization : Centre Hospitalier de l’Université de Montréal

Other organizations : Saint Louis University, Cedars-Sinai Medical Center, Massachusetts General Hospital, Harvard University

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Variable analysis

independent variables

ADR injection

dependent variables

Urinary albumin/creatinine ratio (mg/mmol)
Urinary albumin level (estimated by Coomassie Blue gel staining)
Urinary Ang II /creatinine ratio (pmol/μmol)

control variables

Mice individually housed in metabolic cages

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