Synthesis and Coating of Silver Nanoparticles

Ag nanoparticles were synthesized and coated as described in the Supplementary Information. Coatings around Ag were based on either NeutrAvidin (Ag-NA) protein or PEG-maleimide (Ag-PEG). Amine-reactive dyes were attached to NA nanoparticles for fluorescence microscopy and FACS (e.g. Ag-NA488). Peptides were synthesized with a biotin label for loading into the binding pocket of NA. Free cysteine-containing peptides were prepared as previously described and conjugated to PEG-maleimide.^{14 (link),25 (link)} The etchant was composed of tripotassium hexacyanoferrate (HCF) and sodium thiosulfate pentahydrate (TS) (Sigma), working concentration of 1-10 mM HCF and TS.

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Braun G.B., Friman T., Pang H.B., Pallaoro A., de Mendoza T.H., Willmore A.M., Kotamraju V.R., Mann A.P., She Z.G., Sugahara K.N., Reich N.O., Teesalu T, & Ruoslahti E. (2014). Etchable plasmonic nanoparticle probes to image and quantify cellular internalization. Nature materials, 13(9), 904-911.

Publication 2014

Amine Biotin Dyes Fluorescence microscopy Free cysteine Hexacyanoferrate Maleimide Neutravidin Peptides Protein Sodium thiosulfate pentahydrate

Corresponding Organization :

Other organizations : Sanford Burnham Prebys Medical Discovery Institute, University of California, Santa Barbara, University of Tartu, Discovery Institute

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Variable analysis

independent variables

Coatings around Ag were based on either NeutrAvidin (Ag-NA) protein or PEG-maleimide (Ag-PEG)

dependent variables

Fluorescence microscopy and FACS (e.g. Ag-NA488)

control variables

The etchant was composed of tripotassium hexacyanoferrate (HCF) and sodium thiosulfate pentahydrate (TS) (Sigma), working concentration of 1-10 mM HCF and TS

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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