Comparative Genomic Analysis of Campylobacter concisus

A total of 15 bacterial strains of five species were used in this study, including 11 C. concisus strains and Bacteroides fragilis ATCC 25285, Bacteroides vulgatus ATCC 8482, Enterococcus faecalis ATCC 19433 and Escherichia coli K12 (Bachmann, 1972 (link); Gregory et al., 1978 (link); Moschetti et al., 1998 (link)). Of the 11 C. concisus strains examined, three strains were from patients with CD, four strains from patients with UC, one strain from patient with gastroenteritis and three strains from healthy controls. These 11 C. concisus strains were randomly chosen from C. concisus strains isolated in our previous studies (Zhang et al., 2009 (link), 2010 (link); Mahendran et al., 2011 (link)). C. concisus consists of two genomospecies (GS), among the 11 strains included, five strains were from GS1 and six strains were from GS2 (Mahendran et al., 2015 (link); Chung et al., 2016 (link)). The other bacterial strains were purchased from the American Type Culture Collection (VA, USA). Detailed information of bacterial species and strains used in this study were provided in Table 1.

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Liu F., Ma R., Riordan S.M., Grimm M.C., Liu L., Wang Y, & Zhang L. (2017). Azathioprine, Mercaptopurine, and 5-Aminosalicylic Acid Affect the Growth of IBD-Associated Campylobacter Species and Other Enteric Microbes. Frontiers in Microbiology, 8, 527.

Publication 2017

Bacteroides fragilis Bacteroides vulgatus Enterococcus faecalis

Bacterial Bacteroides fragilis Bacteroides vulgatus Enterococcus faecalis Escherichia coli k12 Gastroenteritis Patient Strains

Corresponding Organization : UNSW Sydney

Other organizations : Prince of Wales Hospital, Sutherland Hospital

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Variable analysis

independent variables

Bacterial species (C. concisus, Bacteroides fragilis, Bacteroides vulgatus, Enterococcus faecalis, Escherichia coli)
C. concisus strains (from patients with CD, UC, gastroenteritis, and healthy controls)
C. concisus genomospecies (GS1 and GS2)

dependent variables

Not explicitly mentioned

control variables

Bacterial strains purchased from American Type Culture Collection

controls

Positive control: Not specified
Negative control: Not specified

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