Annexin V-FITC and PI Staining for Promastigotes

Externalization of phosphatidyl serine on the outer membrane of untreated and JVPH3- and JVPH4-treated promastigotes was measured by the binding of FITC-annexinV and PI using an annexinV staining kit (Invitrogen BioServices India Pvt. Ltd, Bangalore, India). Flow cytometry was carried out for treated and untreated parasites. The gating was done so that the FL-1 channel denotes the mean intensity of FITC-annexinV, whereas the FL-2 channel denotes the mean intensity of PI (Chowdhury et al. 2012 (link)).

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Mishra A., Vinayagam J., Saha S., Chowdhury S., Roychowdhury S., Jaisankar P, & Majumder H.K. (2014). Isobenzofuranone derivatives exhibit antileishmanial effect by inhibiting type II DNA topoisomerase and inducing host response. Pharmacology Research & Perspectives, 2(6), e00070.

Publication 2014

annexinV staining kit

Fitc Flow cytometry Membrane Parasites Phosphatidyl serine

Corresponding Organization : Indian Institute of Chemical Biology

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Variable analysis

independent variables

JVPH3 treatment
JVPH4 treatment

dependent variables

Externalization of phosphatidyl serine on the outer membrane of promastigotes
Mean intensity of FITC-annexinV (FL-1 channel)
Mean intensity of PI (FL-2 channel)

control variables

Untreated promastigotes

controls

Positive control: Not specified
Negative control: Untreated promastigotes

Annotations

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