All yeast strains were grown to greater than 1 × 107 cells/mL in 30 mL minimal media (16 (link)) or YPD (Supplemental Table S1 ). Genomic DNA from each strain was extracted using Qiagen 20/G Genomic tips from ∼1.5 × 109 cells using the manufacturer's protocol.
All genomic DNA was barcoded using Oxford-Nanopore's native barcoding genomic DNA kit (EXP-NBD104), adapters were added using the ligation sequencing kit (SQK-LSK109). The manufacturer's protocol (versions NBE_9065_v109_revB_23May2018 and NBE_9065_V109_revP_14Aug2019) was followed with the following exceptions: incubation times for enzymatic repair step were increased to 15 min. All Agencourt AMPure XP beads were incubated for 30 min at 37°C before elution. Adapter ligation time was increased to 10 min. Multiplexed libraries were loaded on MinION flowcells (FLO-MIN106D R9) and run on a MinION sequencer (MIN-101B).
All genomic DNA was barcoded using Oxford-Nanopore's native barcoding genomic DNA kit (EXP-NBD104), adapters were added using the ligation sequencing kit (SQK-LSK109). The manufacturer's protocol (versions NBE_9065_v109_revB_23May2018 and NBE_9065_V109_revP_14Aug2019) was followed with the following exceptions: incubation times for enzymatic repair step were increased to 15 min. All Agencourt AMPure XP beads were incubated for 30 min at 37°C before elution. Adapter ligation time was increased to 10 min. Multiplexed libraries were loaded on MinION flowcells (FLO-MIN106D R9) and run on a MinION sequencer (MIN-101B).
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