A GeneChip microRNA 4.0 array (Thermo Fisher Scientific) containing probes representing 2588 miRNAs (miRBase release 21; www.mirbase.org accessed on 24 August 2018) was performed according to the protocol described by Felix F. Tainara [55 (link)]. The data analysis was executed using Expression Console software v.4.0.2 (Affymetrix, Santa Clara, CA, USA) for data summarization, normalization, and quality control. To identify significantly deregulated miRNAs, the following parameters were used in each tumor compared to normal tissues: fold change (FC) ≥ 2, p-value < 0.0001 and a false discovery rate (FDR) < 0.05 were used to compare each tumor to normal tissues.
Álvarez-Hilario L.G., Salmerón-Bárcenas E.G., Ávila-López P.A., Hernández-Montes G., Aréchaga-Ocampo E., Herrera-Goepfert R., Albores-Saavedra J., Manzano-Robleda M.D., Saldívar-Cerón H.I., Martínez-Frías S.P., Thompson-Bonilla M.D., Vargas M, & Hernández-Rivas R. (2023). Circulating miRNAs as Noninvasive Biomarkers for PDAC Diagnosis and Prognosis in Mexico. International Journal of Molecular Sciences, 24(20), 15193.
Corresponding Organization : Center for Research and Advanced Studies of the National Polytechnic Institute
Other organizations :
Universidad Nacional Autónoma de México, Universidad Autónoma Metropolitana, Instituto Nacional de Cancerología, Hospital Médica Sur, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán
Expression levels of 2588 miRNAs (miRBase release 21)
control variables
None explicitly mentioned
controls
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