Cytotoxicity of M. mabokeensis Bark Extracts

The cytotoxicity of the different M. mabokeensis Aubrév bark extracts was estimated on IGROV and OVCAR cells lines (American Type Culture Collection) as described by Kohoude et al. [14 (link)], with minor modifications. Cells were distributed in 96-well plates at 3 × 10⁴ cells/well in 100 μL. After that, 100 μL of the corresponding culture medium (DMEM) containing sample at various concentrations was added. Cell growth was estimated by the MTT assay. MTT is a water-soluble tetrazolium salt with a yellow coloration. Metabolically active cells are able to convert the dye to water-insoluble dark blue formazan by reductive cleavage of the tetrazolium ring. The extracts were resolubilized in the DMSO followed by dilution in the buffer, whereby the DMSO does not exceed 1%. Doxorubicin was used as a positive control. The cells activity inhibition percentage was calculated as follows: $\begin{array}{c}\%\text{\hspace{0.17em}inhibition}=100\times \frac{\left(A_{\text{blank}}-A_{\text{sample}}\right)}{A_{\text{blank}}}.\end{array}$