Total RNA was isolated from huPCLS and qPCR analysis was performed as previously described [15 (link),25 (link),36 (link)] using individual TaqMan® Probe-Based Gene Expression Assays (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) selected for proinflammatory cytokines (IL-1β, IL-6, TNFα, and IL-1α), inflammation (COX-2), relevant cytoprotective and antioxidant enzymes (heme oxygenase-1 (HMOX1) and NADPH:quinone oxidoreductase-1 (NQO1), and cell cycle markers (TP53, CDK2, CDKN1A, CDKN2A, CDK4, CDK6, RB, and E2F). Transcript levels of tested genes were normalized to β-Actin.
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