Immunoblot Analysis of Transfected Cells

The gene-transfected DO-11.10 cells and silk solutions from W1, S01, S13, and S15 strains were processed for immunoblot analyses according as described previously^{14 (link)}. After blocking with Blocking One (Nacalai Tesque, Kyoto, Japan) for 1 h at room temperature and incubated with anti-Myc polyclonal antibody (MBL, code no. 562, Nagoya, Japan), anti-β-actin rabbit MAb (Cell Signaling Technology, code no. #4970, Danvers, MA, USA), and anti-FibL polyclonal antibody^{14 (link)}, followed by horseradish peroxidase conjugated anti-rabbit immunoglobulins (Igs) (Dako, code no. P0399, Glostrup, Denmark), and imaged with Chemi-Lumi One L (Nacalai Tesque).