A tetrachloromethane (CCl4)-induced model of cirrhosis was constructed by i.p. injection of 20% CCl4 in olive oil (5 ml/kg, Sinopharm Chemical Reagent, China) into mice at 8 weeks of age for an 8-week period twice weekly. Vehicle control mice were i.p. injected with matching volumes of olive oil following the same regimen.
The short-term DEN-induced model of liver inflammation was constructed by a single i.p. injection of DEN in physiological saline (100 mg/kg) into mice at eight to 12 weeks of age. All mice were sacrificed after 10 days on a standard chow diet. TNF treatment was performed through two i.p. injections of TNF (40 μg/kg) 6 h prior to sacrifice. For in vivo experiments, recombinant murine CCL28 (R&D Systems) or the Akt inhibitor A6730 was i.p. injected (both 50 mg/kg) 6 h prior to sacrifice. Murine CCL28 is a natural ligand-agonist for murine CCR10 14 (link). Vehicle control mice were i.p. injected with matching volumes of physiological saline following the same regimen.
The long-term diethylnitrosamine (DEN)-induced model of inflammation-driven hepatocarcinogenesis was constructed by a single intraperitoneal (i.p.) injection of DEN in physiological saline (15 mg/kg) into mice at 15 days of age. All mice were sacrificed after 9 months on a standard chow diet. After sacrifice, all surface liver tumor nodules were counted and measured via a caliper.
The short-term DEN-induced model of liver inflammation was constructed by a single i.p. injection of DEN in physiological saline (100 mg/kg) into mice at eight to 12 weeks of age. All mice were sacrificed after 10 days on a standard chow diet. TNF treatment was performed through two i.p. injections of TNF (40 μg/kg) 6 h prior to sacrifice. For in vivo experiments, recombinant murine CCL28 (R&D Systems) or the Akt inhibitor A6730 was i.p. injected (both 50 mg/kg) 6 h prior to sacrifice. Murine CCL28 is a natural ligand-agonist for murine CCR10 14 (link). Vehicle control mice were i.p. injected with matching volumes of physiological saline following the same regimen.
The long-term diethylnitrosamine (DEN)-induced model of inflammation-driven hepatocarcinogenesis was constructed by a single intraperitoneal (i.p.) injection of DEN in physiological saline (15 mg/kg) into mice at 15 days of age. All mice were sacrificed after 9 months on a standard chow diet. After sacrifice, all surface liver tumor nodules were counted and measured via a caliper.
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