HFL1 cells were treated with thrombin (1 U/ml, Sigma-Aldrich, St. Louis, MO) for 6 h and then labeled with 10 μM 5-bromo-2’-deoxyuridine (BrdU) (BD Pharmigen, San Jose, CA) for 18 h. Cells were stained for nuclei and BrdU using 4’,6-diamidino-2-phenylindole (DAPI) and anti-BrdU antibody (Cell Signaling, Danvers, MA) [36 (link)]. Results are expressed as the percentage of DAPI-stained cells that were also BrdU-positive.
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