Thrombin-Induced BrdU Incorporation in HFL1 Cells
Corresponding Organization : University of South Florida
Other organizations : Creighton University, University of Nebraska Medical Center, University of South Carolina, University of Pennsylvania
Variable analysis
- Thrombin (1 U/ml, Sigma-Aldrich, St. Louis, MO)
- Percentage of DAPI-stained cells that were also BrdU-positive
- Time of thrombin treatment (6 h)
- Time of BrdU labeling (18 h)
- Staining with DAPI and anti-BrdU antibody (Cell Signaling, Danvers, MA)
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