The colony formation assay was performed as described previously [24 (link)]. Briefly, 1 × 103 SiHa or CaSki cells were plated in each well of a 24-well plate (3524; Corning) with 3 replicates. After culture for 7 days, the cells were washed with PBS and fixed with 100% methanol for 1 h, stained with 0.5% crystal violet and imaged via light microscopy (Leica, Germany) to determine the number of cells.
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