Sepsis Induction in Mice

For sepsis induction, each mouse was given an intraperitoneal (i.p.) injection of 0.5 mL of the fecal slurry solution using a syringe and 27G needle. Depending on the fecal concentrations (45, 90, and 180 mg/mL) used, each mouse therefore received 2, 4, or 8 mg of FIP solution per 1 g body weight respectively. Sham mice were injected with sterile normal saline (NS). Pain (either from the injection or from the fecal slurry)was assessed using facial expression as described by Langford et al.[16 (link)], as well as body posture and vocalization. Analgesia was provided by a subcutaneous injection of buprenorphine (0.1 mg/kg).