AGP N-Glycan Profiling by MALDI-TOF/TOF

AGP preparations were purified from serum samples (n = 10) by an AGP-prep-DOCK^{26 (link)}. N-Glycans of AGP and labeled N-glycans of AGP were prepared and purified as described previously^{26 (link)} using PNGase F (Roche Applied Science, Indianapolis, IN) and BlotGlyco (Sumitomo Bakelite, Co., Tokyo, Japan). Mass spectrometric data were obtained using a MALDI-TOF/TOF ultrafleXtreme (Bruker) and all the primary structures of AGP glycans were assigned with the aid of the AGPAS software^{25 (link),26 (link)}. The relative abundance of α1,3fucosylated tri- and tetraantennary glycans in AGP was defined as FUCAGP^{26 (link)}.

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Yokobori T., Yazawa S., Asao T., Nakazawa N., Mogi A., Sano R., Kuwano H., Kaira K, & Shirabe K. (2019). Fucosylated α1-acid glycoprotein as a biomarker to predict prognosis following tumor immunotherapy of patients with lung cancer. Scientific Reports, 9, 14503.

Publication 2019

PNGase F BlotGlyco MALDI-TOF/TOF ultrafleXtreme

Bakelite Glycans Maldi Mass spectrometric Pngase f Serum

Corresponding Organization : Gunma University

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Variable analysis

independent variables

AGP preparations purified from serum samples (n = 10) by an AGP-prep-DOCK

dependent variables

Relative abundance of α1,3fucosylated tri- and tetraantennary glycans in AGP, defined as FUCAGP

control variables

Preparation and purification of N-Glycans of AGP and labeled N-glycans of AGP using PNGase F and BlotGlyco, as described previously
Mass spectrometric data obtained using a MALDI-TOF/TOF ultrafleXtreme
Primary structures of AGP glycans assigned with the aid of the AGPAS software

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