Lymphoma Cell Line Culturing Protocol

Lymphoma cell lines were cultured according to the recommended conditions, as previously reported [14 (link), 15 (link)]. All media were supplemented with fetal bovine serum (10%), Penicillin‐Streptomycin 100X (Euroclone, ECB3001D). Cell line's identity was confirmed by short tandem repeat (STR) DNA fingerprinting using the Promega GenePrint 10 System kit (B9510) [9 (link)], and all the experiments were performed within 1 month from being thawed. Cells were periodically tested to confirm mycoplasma negativity using the MycoAlert Mycoplasma Detection Kit (Lonza, Visp, Switzerland).

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Spriano F., Sartori G., Tarantelli C., Barreca M., Golino G., Rinaldi A., Napoli S., Mascia M., Scalise L., Arribas A.J., Cascione L., Zucca E., Stathis A., Gaudio E, & Bertoni F. (2022). Pharmacologic screen identifies active combinations with BET inhibitors and LRRK2 as a novel putative target in lymphoma. EJHaem, 3(3), 764-774.

Publication 2022

Penicillin‐Streptomycin 100X GenePrint 10 System kit MycoAlert Mycoplasma Detection Kit

Cell lines Cells Fetal bovine serum Lymphoma Mycoplasma Penicillin Promega Short tandem repeat Streptomycin

Corresponding Organization : Ente Ospedaliero Cantonale

Other organizations : University of Palermo, SIB Swiss Institute of Bioinformatics

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Cell culture conditions (recommended conditions as previously reported)
Cell line identity (confirmed by STR DNA fingerprinting)
Mycoplasma contamination (periodically tested and confirmed negative)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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