Western blotting of total protein lysates was performed as described before44 (link). The following antibodies were used: Opa1 (BD Biosciences) and beta-Actin (Cell Signaling Technologies). Primary incubations were followed by incubation with secondary HRP-conjugated antibodies (Santa Cruz Biotechnology). Blots were revealed using the Biomax MR film (Kodak).
To quantify the total mitochondrial protein amounts, mitochondria were isolated from experimental P14 cells by using the Qproteome Mitochondria Isolation Kit (QIAgen), following the manufacturer instructions. The mitochondria protein extracts were quantified using the Pierce BCA protein kit (Thermo Fisher).
To quantify the total mitochondrial protein amounts, mitochondria were isolated from experimental P14 cells by using the Qproteome Mitochondria Isolation Kit (QIAgen), following the manufacturer instructions. The mitochondria protein extracts were quantified using the Pierce BCA protein kit (Thermo Fisher).
