Liver Triglyceride Colorimetric Assay

Liver triglyceride was measured by homogenizing 100 mg liver tissue in saline and solubilizing lipid with 0.5% sodium deoxycholate. Liver triglyceride was then determined by colorimetric assay (Thermo Fisher Scientific) as performed previously (Vigueira et al., 2014 (link); McCommis et al., 2015 (link)).

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Vigueira P.A., McCommis K.S., Hodges W.T., Schweitzer G.G., Cole S.L., Oonthonpan L., Taylor E.B., McDonald W.G., Kletzien R.F., Colca J.R, & Finck B.N. (2017). The Beneficial Metabolic Effects of Insulin Sensitizers are not Attenuated by Mitochondrial Pyruvate Carrier 2 Hypomorphism. Experimental physiology, 102(8), 985-999.

Publication 2017

colorimetric assay

Assay Colorimetric Lipid Liver Saline Sodium deoxycholate Tissue Triglyceride

Corresponding Organization : Washington University in St. Louis

Other organizations : Metabolic Solutions Development Company (United States), Fraternal Order of Eagles, University of Iowa

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Liver triglyceride

control variables

Saline for homogenizing liver tissue
0.5% sodium deoxycholate for solubilizing lipid

controls

The authors refer to the methods described in previous publications (Vigueira et al., 2014; McCommis et al., 2015), which may have included positive and negative controls, but these are not explicitly mentioned in the given text.

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