Erastin and RSL3 Cytotoxicity Assay

According to the results of the concentration gradient test, we treated U251 or U87MG cells with erastin or RAS-selective lethal 3 (RSL3) for 24 h.

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Li X., Zhang W., Xing Z., Hu S., Zhang G., Wang T., Wang T., Fan Q., Chen G., Cheng J., Jiang X, & Cai R. (2024). Targeting SIRT3 sensitizes glioblastoma to ferroptosis by promoting mitophagy and inhibiting SLC7A11. Cell Death & Disease, 15(2), 168.

Publication 2024

Corresponding Organization : Renji Hospital

Other organizations : State Key Laboratory of Oncogene and Related Genes

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Variable analysis

independent variables

Treatment with erastin
Treatment with RAS-selective lethal 3 (RSL3)

dependent variables

Cell response to erastin treatment
Cell response to RAS-selective lethal 3 (RSL3) treatment

control variables

Cell line (U251 or U87MG)
Duration of treatment (24 h)

controls

No explicit positive or negative controls mentioned

Annotations

This protocol is too short. Unable to provide accurate annotations

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