Measuring Trichoderma reesei Biomass

The biomass of T. reesei grown in TMM + 2% cellulose with and without glutamine was indirectly determined by the amount of intracellular protein [63 (link), 64 (link)]. In brief, harvested mycelia were suspended in 1 M NaOH and incubated for 2 h with frequent vortex. Then the protein concentration of the supernatant of the suspension was determined by the Modified BCA Protein Assay Kit (Sangon Biotech, Shanghai, China). The biomass dry weight was calculated assuming an average content of 0.32 g intracellular protein per g of dry cell mass.

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Pang A.P., Zhang F., Hu X., Luo Y., Wang H., Durrani S., Wu F.G., Li B.Z., Zhou Z., Lu Z, & Lin F. (2021). Glutamine involvement in nitrogen regulation of cellulase production in fungi. Biotechnology for Biofuels, 14, 199.

Publication 2021

Modified BCA Protein Assay Kit

Assay Cellulose G cell G protein Glutamine Intracellular Mycelia Protein Protein g

Corresponding Organization :

Other organizations : Southeast University, Tianjin University, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Center for Excellence in Molecular Plant Sciences

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Variable analysis

independent variables

Presence or absence of glutamine in the TMM + 2% cellulose medium

dependent variables

Intracellular protein content of T. reesei

control variables

TMM + 2% cellulose medium
Incubation time of 2 hours with frequent vortex
Use of Modified BCA Protein Assay Kit (Sangon Biotech, Shanghai, China) to determine protein concentration
Calculation of biomass dry weight assuming an average content of 0.32 g intracellular protein per g of dry cell mass

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