Imaging Tibial Lesions with 99mTc-medronate

^99mTc-medronate was prepared as per the protocol provided by MDP multidose kit (GE Healthcare, The Netherlands). All mice received an intravenous injection with a dose of approximately 37 MBq ^99mTc through the tail vein. Immediately after 1 hpostinjection, images were acquired using a U-SPECT-II/CT (MILabs), as reported previously [15 (link),17 (link),18 ]. The following criteria were used to confirm the tibial lesion formation: a) visual confirmation of ^99mTc-uptake below the growth plate in right tibia compared to contralateral control tibia; b) at least 10% ^99mTc-uptake increase in tibial lesion region of interest (ROI) below growth plate compared to contralateral control tibia.

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Nadar R.A., Franssen G.M., Van Dijk N.W., Codee-van der Schilden K., de Weijert M., Oosterwijk E., Iafisco M., Margiotta N., Heskamp S., van den Beucken J.J, & Leeuwenburgh S.C. (2020). Bone tumor–targeted delivery of theranostic 195mPt-bisphosphonate complexes promotes killing of metastatic tumor cells. Materials Today Bio, 9, 100088.

Publication 2020

U-SPECT-II/CT

99mtc medronate Growth plate Mice Spect ct Tail Tibia Vein

Corresponding Organization : National Research Council

Other organizations : Nuclear Research and Consultancy Group, University of Bari Aldo Moro

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Variable analysis

independent variables

Dose of approximately 37 MBq 99mTc-medronate injected intravenously through the tail vein

dependent variables

99mTc-uptake below the growth plate in right tibia compared to contralateral control tibia
Percentage increase in 99mTc-uptake in tibial lesion region of interest (ROI) below growth plate compared to contralateral control tibia

control variables

Time point of image acquisition (immediately after 1 h post-injection)
Imaging technique (U-SPECT-II/CT)

controls

Contralateral control tibia

Annotations

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